A UV-Visible spectrophotometer is an analytical instrument used to measure the amount of ultraviolet (UV) and visible light absorbed by a sample. It is commonly employed in various scientific fields, including chemistry, biochemistry, and materials science. Here are some key points about UV-Visible spectrophotometers:

• Principle: UV-Visible spectrophotometry is based on the principle that different substances absorb light at specific wavelengths. The spectrophotometer measures the intensity of light before and after it passes through a sample, allowing the determination of the amount of light absorbed.
• Applications: UV-Visible spectrophotometers have a wide range of applications. They are used to analyze the concentration of substances in a solution, determine the purity of compounds, study chemical reactions, and identify unknown substances.
• Wavelength Range: UV-Visible spectrophotometers cover a wavelength range from ultraviolet (typically around 190-400 nm) to visible light (400-700 nm). Some advanced models may extend the range further into the infrared region.
• Components: A typical UV-Visible spectrophotometer consists of a light source, monochromator or prism to select specific wavelengths, a sample holder, and a detector to measure the intensity of transmitted or absorbed light.
• Data Analysis: The data obtained from a UV-Visible spectrophotometer is typically presented as an absorption spectrum, which shows the absorbance of the sample at different wavelengths. The absorbance is related to the concentration of the absorbing substance through Beer-Lambert’s law.

UV-Visible spectrophotometers are valuable tools in scientific research, quality control, and various industries. They provide valuable information about the properties of substances and help in the quantitative and qualitative analysis of samples.